Cytotoxicities of sodium benzoate in primary culture of hepatocytes from adult rat liver.
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Liver regeneration studies with rat hepatocytes in primary culture.
Adult rat parenchymal hepatocytes in primary culture can be induced to enter into DNA synthesis and mitosis. The optimal conditions for hepatocyte replication are low plating density (less than 10,000 cells/sq cm) and 50% serum from two-thirds partially hepatectomized rats (48 hr after hepatectomy). Approximately 80% of the hepatocytes enter the cell cycle, and most of these cells go through mi...
متن کاملPlasminogen binding to rat hepatocytes in primary culture and to thin slices of rat liver.
Human 125I-plasminogen bound readily to rat hepatocytes in primary culture at 4 degrees C and at 37 degrees C. Binding was inhibited by lysine and reversed by lysine, epsilon-aminocaproic acid, or nonradiolabeled plasminogen. The Kd for binding of 125I-plasminogen to hepatocytes was 0.59 +/- 0.16 mumol/L, as determined from the saturation isotherm by nonlinear regression (r2 = 0.99) and the Sca...
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این تحقیق به بررسی استراتژیهای بکاررفته توسط مترجمین مختلف در ترجمه واژه های فرهنگی خاص در ترجمه فارسی به انگلیسی قانون مدنی و قانون اساسی جمهوری اسلامی ایران میپردازد.این تحقیق بر اساس نظریه پیتر نیومارک صرت گرفته است. در این تحفیق واژه های فرهنگی خاص اقتباس شده و پس از مقایسه با متن مبدا به بررسی انها پرداخته می شود.در قسمت ضمائم تحقیق واژه نامه ای برای اشنایی خوانندگان با لغات و اصطلاحات حقو...
Adult rat hepatocytes in primary monolayer culture. Ultrastructural characteristics of intercellular contacts and cell membrane differentiations
Primary monolayer cultures were obtained in 60-mm petri dishes by incubating 3 X 10(6) isolated hepatocytes at 37 degrees C in Dulbecco's medium supplemented with 17% fetal calf serum. The ultrastructure of monolayer cells was examined after various incubation periods. Within 4 h of plating, the isolated spherical cells adhere to the plastic surface, establish their first contacts by numerous i...
متن کاملMaintenance of differentiated rat hepatocytes in primary culture.
Normal adult rat hepatocytes remained viable and functional for at least 43 days when plated on collagen-coated dishes and fed chemically defined medium supplemented with dimethyl sulfoxide (Me2SO). Hepatocytes isolated by collagenase perfusion and cultured in the presence or absence of Me2SO were (i) examined by light and electron microscopy for morphological changes; (ii) analyzed for the pro...
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ژورنال
عنوان ژورنال: The Tohoku Journal of Experimental Medicine
سال: 1987
ISSN: 0040-8727,1349-3329
DOI: 10.1620/tjem.152.47